Wang, M.; He, L.

Tyrosol, a natural polyphenolic compound, has been identified as a potent cellular antioxidant. It mitigates oxidative stress-induced damage in skeletal muscle cells and facilitates the recovery of intracellular adenosine triphosphate (ATP), suggesting a beneficial role in maintaining cellular energy homeostasis. Creatine, widely used to enhance muscle strength by augmenting the phosphagen system, promotes intracellular ATP production but yields relatively modest improvements in endurance. In this study, we investigated the effects of combined supplementation of tyrosol (CreaSol) and creatine monohydrate (CM) on muscle endurance and strength in mice. Following a 4-week exercise training and intragastric intervention, muscle strength and exercise endurance were evaluated through four consecutive forelimb grip strength tests and exhaustive weighted swimming tests at 24-hour intervals. Compared with creatine monohydrate supplementation alone, the co-administration of CreaSol and creatine monohydrate significantly enhanced grip strength (+28.1%) and swimming endurance (+51.5%). More importantly, following consecutive exhaustive exercise, the combined group exhibited superior recovery capacity, demonstrating significantly attenuated declines in both strength and endurance compared to the creatine monohydrate-only group. We conclude that CreaSol not only effectively improves exercise performance in mice, but also significantly enhances the efficacy of creatine monohydrate in improving muscle strength and endurance, as well as reducing fatigue during consecutive high-intensity exercise.